Agarose gel electrophoresis pattern of serum alkaline phosphatase isoenzymes in Holstein cows during lactation

A recent study found that an agarose gel electrophoresis (AGE) method yielded two distinct major bands corresponding to the hepatic and bone ALP isoenzymes (ALP2 ALP3, respec-tively) in bovine serum treated with protease neuraminidase (PN-treatment), although there were concerns intestinal isoenzyme (ALP5) often overlapped ALP3 human neuraminidase. Because ALP5 was separated from alone (P-treatment), we used a modified employing both P- PN-treated sera measure activities of three 53 lacta-ting Holstein cows: 24 primiparous 29 multiparous. Upon electrophoresis, 51 samples (96.2%) subjected P-treatment fraction ALP5, as did control serum. All definite ALP2 fraction. The calculated remainder after excluding ALP5. total (t-ALP) cows higher than those multiparous (p ⟨ 0.001) at early-to-peak [10-110 days milk (DIM)] mid (111-220 DIM) lactation. In multi-parous cows, activity late lactation (221-477 significantly Thus, AGE described here is able discrimi-nate fractions lactating cows. pattern circu-lating will contribute understanding physiological metabolism status